| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 成功大學 | 吳欣儒,李羿亭,劉 樺,黃甜兒 | 04:36.75 | 50M:33.29, 100M:1:09.03, 150M:1:42.42, 200M:2:18.63, 250M:2:51.78, 300M:3:28.60, 350M:4:01.29, 400M:4:36.75 | q01 | |
| 臺灣大學 | 毛文玲,葉兆容,劉月涵,林楷庭 | 04:38.03 | 50M:33.59, 100M:1:09.82, 150M:1:42.32, 200M:2:18.54, 250M:2:51.63, 300M:3:30.03, 350M:4:01.96, 400M:4:38.03 | q02 | |
| 臺灣體大 | 葉璟雯,侯又嘉,陳維青,邱筠文 | 04:38.22 | 50M:31.68, 100M:1:05.49, 150M:1:36.47, 200M:2:11.68, 250M:2:46.02, 300M:3:22.88, 350M:3:57.31, 400M:4:38.22 | q03 | |
| 政治大學 | 毛怡蓁,許乃予,黃敏蓁,徐嘉妤 | 04:40.48 | 50M:32.68, 100M:1:08.99, 150M:1:44.21, 200M:2:26.30, 250M:2:58.88, 300M:3:34.62, 350M:4:05.87, 400M:4:40.48 | q04 | |
| 淡江大學 | 黃琪淇,黃心垚,陳方捷,莊博雅 | 04:41.83 | 50M:33.69, 100M:1:12.64, 150M:1:46.19, 200M:2:22.74, 250M:2:57.00, 300M:3:35.19, 350M:4:06.22, 400M:4:41.83 | q05 | |
| 臺北大學 | 莫倩欣,劉子瑜,陳紫妶,黃子嫣 | 04:43.15 | 50M:32.28, 100M:1:07.94, 150M:1:42.70, 200M:2:21.86, 250M:2:54.93, 300M:3:30.98, 350M:4:04.42, 400M:4:43.15 | q06 | |
| 臺北商大 | 陳榕姿,藍千妮,粘妤甄,劉祐綺 | 04:45.52 | 50M:33.48, 100M:1:09.76, 150M:1:44.63, 200M:2:24.48, 250M:2:58.02, 300M:3:37.80, 350M:4:10.64, 400M:4:45.52 | q07 | |
| 臺灣師大 | 楊晨希,臺芷佑,周筠筑,羅誼恩 | 04:49.36 | 50M:32.70, 100M:1:08.10, 150M:1:42.37, 200M:2:20.82, 250M:2:54.91, 300M:3:33.47, 350M:4:09.21, 400M:4:49.36 | q08 | |
| 中央大學 | 郭郁炩,洪琬哲,林芸安,藍心妤 | 04:51.40 | 50M:33.96, 100M:1:10.16, 150M:1:44.25, 200M:2:24.17, 250M:2:59.54, 300M:3:37.84, 350M:4:12.07, 400M:4:51.40 | | |
| 逢甲大學 | 陳柏羽,楊宥竹,蕭屹峮,馬家祺 | 05:03.47 | 50M:34.21, 100M:1:14.76, 150M:1:47.86, 200M:2:28.97, 250M:3:04.04, 300M:3:46.98, 350M:4:23.11, 400M:5:03.47 | | |
| 東海大學 | 邱鈺暄,陳冠妤,劉芸安,李子芊 | 05:07.09 | 50M:35.11, 100M:1:18.74, 150M:1:57.92, 200M:2:43.49, 250M:3:15.84, 300M:3:54.56, 350M:4:26.02, 400M:5:07.09 | | |
| 陽明交大 | 林家伊,千年倫子,陳譔羽,劉家妤 | 05:08.26 | 50M:35.48, 100M:1:16.65, 150M:1:53.02, 200M:2:33.39, 250M:3:13.40, 300M:3:57.25, 350M:4:30.59, 400M:5:08.26 | | |
| 清華大學 | 葉芯彤,蕭永千,陳柏巧,賴思妤 | 03:42.87 | 50M:45.21, 100M:., 150M:., 200M:3:42.87, 250M:., 300M:., 350M:., 400M:. | | 違規(未完賽，第一棒游50M) |
| 輔仁大學 | 李靜提,王喬盈,林韋彤,吳美錞 | | 50M:36.57, 100M:1:17.14, 150M:1:53.61, 200M:2:38.36, 250M:3:18.18, 300M:4:00.19, 350M:4:36.49, 400M:5:17.43 | | 逾時 |
| 東華大學 | 潘芷若,劉宸蓁,藍苡瑄,陳泳佑 | | 50M:42.38, 100M:1:31.64, 150M:2:11.31, 200M:2:57.02, 250M:3:33.39, 300M:4:14.06, 350M:4:46.50, 400M:. | | 逾時 |
| 臺北科大 | 邱婕恩,張茹庭,連岑恩,劉予安 | | 50M:31.86, 100M:1:07.25, 150M:1:42.72, 200M:2:22.42, 250M:3:02.25, 300M:3:55.46, 350M:4:34.36, 400M:5:18.31 | | 逾時 |
| 臺北醫大 | 朱村恩,許舒茵,曾欣雲,李沅宸 | | 50M:38.65, 100M:1:25.26, 150M:2:05.09, 200M:2:53.38, 250M:3:32.40, 300M:4:17.59, 350M:5:00.76, 400M:. | | 逾時 |