| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 陽明交大 | 戴伊晨,方淳浲,李宥彤,呂沁恩 | 04:19.47 | 50M:32.82, 100M:1:08.94, 150M:1:41.82, 200M:2:18.64, 250M:2:49.46, 300M:3:24.89, 350M:3:51.04, 400M:4:19.47 | q01 | |
| 北市大學 | 張恪榕,陳仲澤,楊宇晴,劉姵吟 | 04:24.23 | 50M:28.69, 100M:59.30, 150M:1:30.82, 200M:2:07.40, 250M:2:38.88, 300M:3:15.39, 350M:3:48.19, 400M:4:24.23 | q02 | |
| 臺灣師大 | 洪子恩,林廷翰,羅予訢,鄭雲禎 | 04:25.73 | 50M:30.41, 100M:1:03.33, 150M:1:36.15, 200M:2:14.33, 250M:2:44.66, 300M:3:20.99, 350M:3:51.20, 400M:4:25.73 | q03 | |
| 臺灣大學 | 陳思綺,林育緯,卓承齊,李怡諄 | 04:27.99 | 50M:33.94, 100M:1:11.95, 150M:1:44.04, 200M:2:20.61, 250M:2:49.44, 300M:3:20.64, 350M:3:52.24, 400M:4:27.99 | q04 | |
| 臺灣體大 | 蘇聖睎,陳冠吟,張瑞宏,蕭尹婷 | 04:28.97 | 50M:29.02, 100M:1:00.44, 150M:1:38.42, 200M:2:23.18, 250M:2:51.21, 300M:3:23.23, 350M:3:54.14, 400M:4:28.97 | q05 | |
| 國北教大 | 謝宜臻,江承恩,許鈞翔,翁郁姍 | 04:30.14 | 50M:34.23, 100M:1:13.32, 150M:1:45.41, 200M:2:21.88, 250M:2:49.58, 300M:3:22.69, 350M:3:54.49, 400M:4:30.14 | q06 | |
| 海洋大學 | 李天曦,蘇柏綸,李玥彤,廖涵芸 | 04:31.06 | 50M:30.16, 100M:1:02.60, 150M:1:36.39, 200M:2:13.80, 250M:2:45.78, 300M:3:24.75, 350M:3:55.93, 400M:4:31.06 | q07 | |
| 輔仁大學 | 艾晴天,石蕓瑈,彭柏深,高學澤 | 04:35.08 | 50M:34.13, 100M:1:12.00, 150M:1:52.25, 200M:2:37.87, 250M:3:05.66, 300M:3:39.11, 350M:4:05.84, 400M:4:35.08 | q08 | |
| 虎尾科大 | 蔡宸洋,陳科均,林芯宇,江珮歆 | 04:35.72 | 50M:30.99, 100M:1:05.59, 150M:1:38.59, 200M:2:18.25, 250M:2:50.77, 300M:3:29.48, 350M:4:00.25, 400M:4:35.72 | | |
| 國立體大 | 王安安,曾楷文,廖梓丞,張芸慈 | 04:40.22 | 50M:37.45, 100M:1:19.15, 150M:1:51.34, 200M:2:29.19, 250M:2:57.45, 300M:3:29.84, 350M:4:02.51, 400M:4:40.22 | | |
| 中國醫大 | 林怡亭,王云楨,彭致豪,胡紳宏 | 04:42.65 | 50M:34.13, 100M:1:14.68, 150M:1:53.42, 200M:2:39.75, 250M:3:08.62, 300M:3:43.47, 350M:4:11.34, 400M:4:42.65 | | |
| 義守大學 | | | | | 請假 |
| 中正大學 | 王韻淇,董俊佑,張睿晨,楊雅婷 | | | | 請假 |