| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 北市大學 | 何亞璇,洪潔瑜,劉姵吟,李宜親 | 04:37.94 | 50M:34.83, 100M:1:11.83, 150M:1:46.94, 200M:2:26.73, 250M:2:57.67, 300M:3:34.07, 350M:4:03.99, 400M:4:37.94 | q01 | |
| 陽明交大 | 戴伊晨,陳瑀婕,李宥彤,謝沂芹 | 04:39.36 | 50M:32.95, 100M:1:09.39, 150M:1:46.57, 200M:2:30.31, 250M:3:01.51, 300M:3:37.29, 350M:4:07.03, 400M:4:39.36 | q02 | |
| 臺灣體大 | 林佩欣,羅羽廷,陳思安,陳冠吟 | 04:41.28 | 50M:34.58, 100M:1:13.67, 150M:1:50.52, 200M:2:31.70, 250M:3:01.42, 300M:3:36.74, 350M:4:08.08, 400M:4:41.28 | q03 | |
| 臺灣師大 | 陳庭萱,季瀠瀠,羅予訢,鄭雲禎 | 04:46.57 | 50M:34.85, 100M:1:11.68, 150M:1:49.81, 200M:2:34.66, 250M:3:05.13, 300M:3:42.06, 350M:4:12.06, 400M:4:46.57 | q04 | |
| 清華大學 | 王怡淨,黃沛儀,張妃婷,莊宥綺 | 04:50.09 | 50M:35.31, 100M:1:13.68, 150M:1:51.48, 200M:2:34.09, 250M:3:06.60, 300M:3:42.76, 350M:4:14.55, 400M:4:50.09 | q05 | |
| 輔仁大學 | 艾晴天,石蕓瑈,林姿婷,呂芝妍 | 05:01.76 | 50M:34.20, 100M:1:11.81, 150M:1:51.95, 200M:2:37.66, 250M:3:12.45, 300M:3:54.69, 350M:4:26.49, 400M:5:01.76 | q06 | |
| 海洋大學 | 黎青真,廖涵芸,李玥彤,周妤倢 | 05:04.20 | 50M:37.52, 100M:1:17.30, 150M:1:58.19, 200M:2:45.06, 250M:3:18.24, 300M:3:58.02, 350M:4:29.39, 400M:5:04.20 | q07 | |
| 中正大學 | 張奕涵,張雅筑,王韻淇,楊雅婷 | 05:06.39 | 50M:34.17, 100M:1:15.05, 150M:1:57.70, 200M:2:45.87, 250M:3:17.88, 300M:3:57.90, 350M:4:29.58, 400M:5:06.39 | q08 | |
| 中國醫大 | 林怡亭,蔡兆衣,王云楨,何翊玄 | 05:06.69 | 50M:34.03, 100M:1:13.99, 150M:1:53.11, 200M:2:39.19, 250M:3:13.93, 300M:3:56.71, 350M:4:29.04, 400M:5:06.69 | | |
| 國北教大 | 謝宜瑾,蕭伃涵,謝宜臻,翁郁姍 | 05:19.74 | 50M:35.04, 100M:1:14.85, 150M:2:00.24, 200M:2:50.30, 250M:3:25.30, 300M:4:09.76, 350M:4:42.73, 400M:5:19.74 | | |
| 高雄師大 | | | | | 請假 |
| 臺灣科大 | | | | | 請假 |