| 單 位 | 姓 名 | 成 績 | 分段時間 | 晉 級 | 備 註 |
| 北市大學 | 邱羿臻,陳妤函,洪潔瑜,何亞璇 | 04:16.04 | 50M:28.00, 100M:1:00.62, 150M:1:29.94, 200M:2:02.69, 250M:2:36.10, 300M:3:11.34, 350M:3:43.25, 400M:4:16.04 | q01 | |
| 清華大學 | 王怡淨,莊宥綺,黃沛儀,張妃婷 | 04:16.70 | 50M:28.96, 100M:1:01.16, 150M:1:32.93, 200M:2:07.79, 250M:2:38.54, 300M:3:13.44, 350M:3:44.10, 400M:4:16.70 | q02 | |
| 臺灣師大 | 羅予訢,葉亞芯,詹庭瑜,鄭雲禎 | 04:16.77 | 50M:29.67, 100M:1:03.56, 150M:1:32.98, 200M:2:06.77, 250M:2:38.51, 300M:3:13.24, 350M:3:42.73, 400M:4:16.77 | q03 | |
| 臺灣體大 | 蕭尹婷,林洵雯,鄒瑋倫,陳俞蓉 | 04:20.69 | 50M:32.71, 100M:1:07.72, 150M:1:37.54, 200M:2:10.93, 250M:2:43.10, 300M:3:19.45, 350M:3:48.45, 400M:4:20.69 | q04 | |
| 陽明交大 | 戴伊晨,朱莉穎,陳瑀婕,謝沂芹 | 04:23.54 | 50M:30.57, 100M:1:03.12, 150M:1:33.66, 200M:2:08.70, 250M:2:41.74, 300M:3:18.34, 350M:3:49.21, 400M:4:23.54 | q05 | |
| 海洋大學 | 廖涵芸,黎青真,周妤倢,李玥彤 | 04:23.69 | 50M:31.76, 100M:1:06.16, 150M:1:38.42, 200M:2:12.25, 250M:2:43.43, 300M:3:18.83, 350M:3:49.67, 400M:4:23.69 | q06 | |
| 高雄師大 | 王如庭,林詠心,蔡采潔,李芯愛 | 04:27.74 | 50M:30.41, 100M:1:02.88, 150M:1:34.50, 200M:2:08.95, 250M:2:41.40, 300M:3:20.73, 350M:3:52.17, 400M:4:27.74 | q07 | |
| 臺灣科大 | 徐芳澄,呂治慧,陳子安,謝語磬 | 04:29.29 | 50M:32.81, 100M:1:06.90, 150M:1:37.33, 200M:2:09.40, 250M:2:42.93, 300M:3:18.57, 350M:3:52.89, 400M:4:29.29 | q08 | |
| 國北教大 | 謝宜臻,翁郁姍,胡 立,謝宜瑾 | 04:30.16 | 50M:31.87, 100M:1:06.64, 150M:1:38.81, 200M:2:14.15, 250M:2:46.98, 300M:3:22.70, 350M:3:54.45, 400M:4:30.16 | | |
| 輔仁大學 | 石蕓瑈,艾晴天,林姿婷,呂芝妍 | 04:33.17 | 50M:31.99, 100M:1:07.54, 150M:1:39.67, 200M:2:14.82, 250M:2:48.39, 300M:3:26.49, 350M:3:58.05, 400M:4:33.17 | | |
| 中國醫大 | 林怡亭,蔡兆衣,何翊玄,王云楨 | 04:41.19 | 50M:29.81, 100M:1:08.48, 150M:1:40.48, 200M:2:18.04, 250M:2:51.13, 300M:3:29.02, 350M:4:02.57, 400M:4:41.19 | | |